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Optimisation of the cultured ELISpot/Fluorospot technique for the selective investigation of SARS-CoV-2 reactive central memory T cells

This study presents an optimised cultured ELISpot protocol for detecting central memory T-cell interferon gamma (IFNγ) responses against SARS-CoV-2 peptides following an initial priming with either peptides, or whole spike protein. 

Citation:
Jerome JR, Wilson KL, Fialho J, …….  McLeod C, Richmond PC, Apostolopoulos V, et al.  Optimisation of the cultured ELISpot/Fluorospot technique for the selective investigation of SARS-CoV-2 reactive central memory T cells. Front Immunol. 2025;16:1547220.

Keywords:
COVID; COVID-19; ELISpot; Fluorospot; SARS-CoV-2; central memory T-cell responses; immune responses; method.

Abstract:
This study presents an optimised cultured ELISpot protocol for detecting central memory T-cell interferon gamma (IFNγ) responses against SARS-CoV-2 peptides following an initial priming with either peptides, or whole spike protein.