HPLC-UV assay of tramadol and O-desmethyltramadol in human plasma containing other drugs potentially co-administered to participants in a paediatric population pharmacokinetic study

Multimodal analgesia is employed in paediatric pain management to maximise analgesia and minimise side effects. Tramadol is dosed at 1–1.5 mg/kg to treat severe pain in children but the assay for tramadol in plasma samples for pharmacokinetic and toxicology studies does not often consider concurrently administered medications.

Citation:
Yoo O, Tang EKY, Nguyen MN, Salman S, Hua AJ, von Ungern Sternberg BS, et al. HPLC-UV assay of tramadol and O-desmethyltramadol in human plasma containing other drugs potentially co-administered to participants in a paediatric population pharmacokinetic study. J Chromatogr B Anal Technol Biomed Life Sci. 2021;1184.

Keywords:
HPLC assay; Paediatrics pharmacokinetics; plasma extraction; Population pharmacokinetics; Tramadol; Drug interactions; Pediatrics

Abstract:
Multimodal analgesia is employed in paediatric pain management to maximise analgesia and minimise side effects. Tramadol is dosed at 1–1.5 mg/kg to treat severe pain in children but the assay for tramadol in plasma samples for pharmacokinetic and toxicology studies does not often consider concurrently administered medications.